Identification and Analysis of Active Carotenoid Compounds From Entawak Fruit (Artocarpus Anisophyllus)

Gervacia Jenny Ratnawaty, Ratih Indrawati, Jajar Pramata Syari

Abstract


— Isolation and identification of active compounds from mentawak fruit (Artocarpus Anisophyllus) used a plant determination test to determine the plant content. Furthermore, the extraction process was carried out to obtain a crude extract of carotenoid pigments by maceration method using n-hexane as solvent. Furthermore, the obtained macerate was carried out by phytochemical tests to determine the content of the active compounds. The TLC test was carried out to confirm the presence of a positive group of compounds on phytochemical screening, and to determine the chromatographic profile of the extract.  The identification results showed that the phytochemicals showed that the active compounds contained in macerate were alkaloids, flavonoids, tannins and saponins. The KLT test results obtained an Rf value of 0.875. While the total carotenoid content using a spectrophotometer UV-Vis is 958 µg/ml.

Keywords


active compounds, Artocarpus anisophyllus, thin layer chromatography (TLC), Spectrophotometer UV-Vis

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References


W. Merdekawati, F. F. Karwur, and A. B. Susanto, “Karotenoid Pada Algae: Kajian Tentang Biosintesis, Distribusi Serta Fungsi Karotenoid,” Bioma, vol. 13, no. 1, pp. 23–32, 2017, doi: 10.21009/bioma13(1).3.

L. H. Endarini, Farmakognisi Dan Fitokimia, 1st ed. Jakarta: Pusdik SDM Kesehatan Kementrian Kesehatan RI, 2016.

S. Koswara, “Pewarna Alami: Produksi dan Pengolahannya,” 2009, [Online]. Available: http://tekpan.unimus.ac.id/wp-content/uploads/2013/07/Pewarna Alami.pdf.

BPTP and (Badan Penelitian Dan Pengembangan Pertanian) Kementrian Pertanian Pertanian Kal-Bar, “Penelitian Mentawak ( Artocarpus anisophyllus).” 2019.

A. Hakim, “Diversity of secondary metabolites from Genus Artocarpus ( Moraceae ),” J. Nusant. Biosci., vol. 2, no. 3, pp. 146–156, 2010, doi: 10.13057/nusbiosci/n020307.

H. Chadburn, “Artocarpus anisophyllus,” IUCN Red List Threat. SpeciesTM, vol. 8235, pp. 301–303, 2018, doi: 10.1007/978-94-007-2534-8_38.

H. S. Maleta, R. Indrawati, L. Limantara, T. Hardo, and P. Brotosudarmo, “Ragam Metode Ekstraksi Karotenoid dari Sumber Tumbuhan dalam Dekade Terakhir ( Telaah Literatur ) Various Carotenoid Extraction Methods from Sources of Plants in Recent Decade ( Review Paper ),” J. Rekayasa Kim. dan Lingkung., vol. 13, no. 1, 2018.

S. dkk Allen, “Process For Extracting Carotenoids From Fruit and Vegetables Processing Waste,” US 2004O131748A1, vol. 1, no. 19, 2004.

J. Ngginak, A. Rafael, D. Amalo, S. T. Nge, and C. L. Sandra Bisilissin, “Analisis Kandungan Senyawa β-Karoten Pada Buah Enau (Arenga piñata) Dari Desa Baumata " Jambura Edu Biosf. J., vol. 2, no. 1, pp. 2656–0526, 2020, [Online]. Available: http://ejurnal.ung.ac.id/index.php/edubiosfer/article/view/2717.

Mufadal, “Isolasi Senyawa Alkaloid dari Alga Merah menggunakan KLT serta serta analisa dengan Spektrofotometer uv-vis dan FTIR,” 2015.

M. Mulana, Profil Kromatografi Lapis Tipis (KLT) Ekstrak Daun Bidara Arab ( Ziiphus spina cristi.L) Berdasarkan Variasi Pelarut. 2018, p. 2018.




DOI: http://dx.doi.org/10.31963/intek.v8i2.2984

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