ISOLASI SENYAWA FLAVONOID DARI KAYU SEPANG (CAESALPINIA SAPPAN L.) DENGAN METODE ULTRASONIC ASSISTED SOLVENT EXTRACTION DAN KARAKTERISASINYA DENGAN METODE GAS CHROMATOGRAPHY MASS SPECTROMETRY (GCMS)

Abstrak

Sappan wood is a source of natural antioxidants. Efficacy of Sappan plants as antioxidants also as antimicrobialsand natural coloring agents. The ability of treatment with the use of Sappan plants is caused by the abundance ofchemical compounds in plants such as the presence of flavonoids. Various methods of preparative analysis have beenfound and developed to isolate flavonoid compounds, one of which is done by extracting using Ultrasonic AssistedSolvent Extraction using variations of ethanol solvent concentration of 85%, 90%, 95%, and 99%. The highestpercentage of yield obtained at 90% ethanol concentration with a time of 15 minutes is 0.42% and pH is 5. The results ofGCMS analysis identified components of phenol compounds which are flavonoid compounds with 2-Methyl-5- (1,2,2-Trimethylcyclopentyl) Phenol with% Area is 44.56% at 90% ethanol concentration with 15 minutes extraction time,where the longer extraction time of flavonoid components will decrease due to degradation into other compounds.