PEMANFAATAN LIMBAH ALGINAT MENJADI GULA REDUKSI MELALUI HIDROLISIS MENGGUNAKAN ENZIM SELULASE

Abstract

The use of brown seaweed for the industry is mainly based on the chemical content contained in seaweed such as
alginate, jelly, and carrageenan. The main component of seaweed is carbohydrates. Hydrolysis is a process carried out to
make polysaccharide molecules into simple sugars such as glucose and galactose. This study aims to look at the effect of
enzyme concentration and incubation time on the hydrolysis of alginate waste substrates using cellulase enzymes. The
process of hydrolysis of alginate waste flour produces sugar content, which was previously carried out by ultrasonic
pretreatment. The hydrolysis process uses cellulase enzymes with various enzyme concentrations (8, 10, 12, 14 and 16 U/mg)
in a 7% substrate and variations in incubation time (84, 96 and 108 hours). Analysis of reducing sugar content using a UVVisible
Spectrophotometer with a wavelength of 575 nm. The highest reducing sugar content was obtained at 96 hours with
an additional concentration of 12 U/mg enzyme (6.55%).