PERBANDINGAN PERTUMBUHAN JAMUR PADA MEDIA BEKATUL DEXTROSE AGAR (BDA) DAN POTATO DEXTROSE AGAR (PDA)

Abstract

Fungal culture media in laboratory containing high carbohydrate source, nitrogen source are required for the growth. This
nutrient can be found in bran that contains high carbohydrates, proteins, fats, vitamins, and crude fiber. So that bran can
be used as raw material for alternative fungal growth media. The aim of this study was to compare fungal growth on bran
infusion dextrose agar (BDA) and potato dextrose agar (PDA). Mix bran infusion with dextrose, agar and water and boil
to dissolve. PDA used as the standard. Plugs of spores of Aspergillus niger placed at the centre of each petri plate were
used to assess growth and sporulation. Candida albicans is inoculated on BDA and PDA using the spread plate method.
The mycelial growth and sporulation of A. niger on all media were reasonably good. There was no significant difference
(P > 0.05) between colony diameters and sporulation. Colonies of Candida albicans on BDA and PDA were 8.5x105
CFU and 8.5x105 CFU. The bran media in the study supported growth and sporulation of the test fungi.