KARAKTERISTIK FENOTIP DAN GENOTIP MIKROSIMBION SPONS NIPHATES SP SEBAGAI BIOMATERIAL POTENSIAL PENDEGRADASI POLI AROMATIK HIDROKARBON

Abstract

Sponges are often used as a reference for pollutant bioindicators, (pollution of heavy metals and poly aromatichydrocarbons. Porosity of the sponge body is used as host microorganisms, especially bacteria and fungi. Phenotypiccharacterization and symbiotic genotypes of Niphates sp. molecular rRNA gene 2 of Nephates sp as symbionts isolatesshowed a positive reaction to methyl red (acid producer), VR, citrate (has a genetic carrier enzyme), lactose and catalase(acidic). This data shows that both isolates of Niphates sp can make carbon as a nutrient source of energy: Genotypicanalysis of 2 isolates of Nephates sp in the phases of amplification and alignment of the sample gene homolog series onthe Bank gene concluded that the 2 isolates were Bacillus cohnii strain DSM 6307 (BC) and Bacillus pumilus strainGLB197 (BP). (Fenantrena) shows growth cell cells based on parameters of increased optical density, gas bubblesformed, changes in media pH and fermentation odor arises.