PENGEMBANGAN METODE NIR SPEKTROSKOPI UNTUK DETEKSI CEPAT KADAR KAFEIN DALAM KOPI

Abstract

Coffee drinks are very popular because they can relieve fatigue and drowsiness, increase concentration, improve mood, and reduce the risk of depression. However, consuming coffee can have negative effects on the body. The caffeine content in coffee, if consumed excessively can cause headaches, stomach and digestive disorders, and interfere with heart function, which results in increased blood pressure. Caffeine in coffee can be determined using UV VIS Spectrophotometry or the Chromatography method. These methods take a long time and use a lot of organic solvents. This study aims to develop a NIR spectrometry method to detect caffeine levels in coffee quickly. The study began by reading the spectrum of several coffee samples, then caffeine analysis was carried out using the reference method. Data from the spectrum and values from the reference method were processed with NirCal Chemometric Buchi software to produce a calibration model. From the calibration process, the standard error calibration (SEC) value was obtained as 0.1459 and the determination coefficient (R2) as 0.858. This study shows that the results of caffeine analysis use the NIR method and the reference method are not much different, so the NIR method can be used for routine caffeine analysis in the laboratory. Keywords: Coffee, Caffeine, NIR Spectroscopy, Method Development